First Lady is the unofficial title used in some countries for the spouse of an elected head of state.
First Lady may also refer to:
- First Lady (play), a 1935 play by George S. Kaufman and Katharine Dayton
- First Lady (film), a 1937 adaptation of the play
- First Lady (album), an album by Lisa Maffia
- The First Lady, an album by Faith Evans
- The First Lady (TV series), a 1960s British drama series