Protein-coding gene in the species Homo sapiens
ESCRT-II complex subunit crystal structure of subunit vps25 of the endosomal trafficking complex escrt-ii
Symbol ESCRT-II Pfam PF05871 InterPro IPR008570
Vacuolar protein-sorting-associated protein 25 is a protein that in humans is encoded by the VPS25 gene .[5] [6]
It is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT (ESCRT-I, -II, -III) complexes orchestrate efficient sorting of ubiquitinated transmembrane receptors to lysosomes via multivesicular bodies (MVBs).[7] ESCRT-II recruits the transport machinery for protein sorting at MVB.[8] In addition, the human ESCRT-II has been shown to form a complex with RNA polymerase II elongation factor ELL in order to exert transcriptional control activity. ESCRT-II transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.[9]
ESCRT-II is a trilobal complex composed of two copies of vps25, one copy of vps22 and the C-terminal region of vps36 . The crystal structure of vps25 revealed two winged-helix domains, the N-terminal domain of vps25 interacting with vps22 and vps36.[10]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000131475 – Ensembl , May 2017
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000078656 – Ensembl , May 2017
^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Yorikawa C, Shibata H, Waguri S, Hatta K, Horii M, Katoh K, Kobayashi T, Uchiyama Y, Maki M (Mar 2005). "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting" . Biochem J . 387 (Pt 1): 17–26. doi :10.1042/BJ20041227 . PMC 1134928 . PMID 15511219 .
^ "Entrez Gene: VPS25 vacuolar protein sorting 25 homolog (S. cerevisiae)" .
^ Gill DJ, Teo H, Sun J, Perisic O, Veprintsev DB, Emr SD, Williams RL (January 2007). "Structural insight into the ESCRT-I/-II link and its role in MVB trafficking" . EMBO J . 26 (2): 600–12. doi :10.1038/sj.emboj.7601501 . PMC 1783442 . PMID 17215868 .
^ Teo H, Perisic O, Gonzalez B, Williams RL (October 2004). "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes" . Dev. Cell . 7 (4): 559–69. doi :10.1016/j.devcel.2004.09.003 . PMID 15469844 .
^ Babst M, Katzmann DJ, Snyder WB, Wendland B, Emr SD (August 2002). "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body" . Dev. Cell . 3 (2): 283–9. doi :10.1016/S1534-5807(02)00219-8 . PMID 12194858 .
^ Wernimont AK, Weissenhorn W (December 2004). "Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II" . BMC Struct. Biol . 4 (1): 10. doi :10.1186/1472-6807-4-10 . PMC 539351 . PMID 15579210 .
Further reading
Kamura T, Burian D, Khalili H, et al. (2001). "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose" . J. Biol. Chem . 276 (19): 16528–33. doi :10.1074/jbc.M010142200 . PMID 11278625 .
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 . PMC 139241 . PMID 12477932 .
von Schwedler UK, Stuchell M, Müller B, et al. (2003). "The protein network of HIV budding" . Cell . 114 (6): 701–13. doi :10.1016/S0092-8674(03)00714-1 . PMID 14505570 . S2CID 16894972 .
Martin-Serrano J, Yarovoy A, Perez-Caballero D, et al. (2003). "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins" . Proc. Natl. Acad. Sci. U.S.A . 100 (21): 12414–9. Bibcode :2003PNAS..10012414M . doi :10.1073/pnas.2133846100 . PMC 218772 . PMID 14519844 .
Sharma M, Pampinella F, Nemes C, et al. (2004). "Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes" . J. Cell Biol . 164 (6): 923–33. doi :10.1083/jcb.200312018 . PMC 2172283 . PMID 15007060 .
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 . PMID 15489334 .
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature . 437 (7062): 1173–8. Bibcode :2005Natur.437.1173R . doi :10.1038/nature04209 . PMID 16189514 . S2CID 4427026 .
Bowers K, Piper SC, Edeling MA, et al. (2006). "Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII" . J. Biol. Chem . 281 (8): 5094–105. doi :10.1074/jbc.M508632200 . PMID 16371348 .