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Since you won't let me respond to or edit the comments made @ http://en.wikipedia.org/wiki/User_talk:Samsara/Archive04 about me as a sign of protest I will be pasting the UMASS/sterilization audio page http://www.worcestermagazine.org/audio.pdf on any wikipedia website where UMASS or Craig Mello are mentioned. |
Since you won't let me respond to or edit the comments made @ http://en.wikipedia.org/wiki/User_talk:Samsara/Archive04 about me as a sign of protest I will be pasting the UMASS/sterilization audio page http://www.worcestermagazine.org/audio.pdf on any wikipedia website where UMASS or Craig Mello are mentioned. |
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Opabinia regalis: You have yet to reveal your identity; I am still waiting. Just your assertion alone that you are not affiliated with UMASS is not worthy of credence. For the last time, who are You? why are you afraid to tell your readers who you really are? |
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==Question== |
==Question== |
Revision as of 15:49, 30 September 2006
The article should explain which organisms use RNAi. All eukaryotes? Only eukaryotes? AxelBoldt 02:38, 12 February 2006 (UTC)
RNAi is due to a conserved mechanism in nematodes, insects, plants and mammals. These are the organisms I know of, not sure of others. Xanin 19:24, 17 April 2006 (UTC)
Removed Link Directory Spam Bioinformin
Knockout
I do not like the use of the word "knockout" to describe RNAi in this article:
- RNAi has recently been applied as an experimental technique to "knockout" genes in model organisms for experimental analysis in determining the function of a gene.
RNAi is not knockout. I have only ever heard knockout used to describe permanent genetic modification. Knockdown is the term that is used for RNA interference universally. Peter Znamenskiy 19:18, 7 June 2006 (UTC)
- Completely agree - knockdown is correct terminology Goldfinger820 03:57, 8 June 2006 (UTC)
You are absolutely correct. Nobody uses the word "knockout" to describe RNAi.
Merge
I was under the impression that RNAi, antisense RNA and miRNA too are all different things (with overlap of course). --Rajah 20:47, 27 June 2006 (UTC)
Here's a good reference showing the two are different: [1]. I don't mind if the articles are merged, I just think that it should be pointed out that antisense RNA and RNAi aren't necessarily the same thing. --Rajah 21:05, 27 June 2006 (UTC)
- RNAi is a completely different biological pathway. Although the down stream mechanism of silencing in both antisense and RNAi involve sequence-specific post transcriptional targeting of mRNA for degradation, the celluar machinary used to trigger them are wholly disimillar — Preceding unsigned comment added by 129.81.15.58 (talk • contribs) 14:14, 19 July 2006 (UTC)
rnaiconception.com
That is not an appropriate link for this page, and repeatedly restoring it without discussion is inappropriate as well. The link is to a personal advocacy site related to an obscure authorship dispute that is irrelevant to the general discussion of RNAi presented here, and the inclusion of only one disputant's opinion on the matter is a violation of the neutral point of view policy. To the anonymous user who has repeatedly inserted this link, please do not do so again, especially not without discussion. Opabinia regalis 23:48, 21 July 2006 (UTC)
I disagree. I think to call the site an "obscure authorship" dispute is dishonest. The author of "RNA interference page" credits and singles out Craig Mello and Andrew Fire as the discoverers of RNAi by name, and yet when a link is presented to the readers with the possibility that Craig Mello and Andrew Fire may have committed an intellectual fraud and thievery to credit themselves as the conceivers of RNAi (based on their own inconsistent stories of conception; the entire website is dedicated to what led Mello et al and Fire et al to the recognition that the key interfering agent mediating RNAi was/is indeed dsRNA molecule, not about authorship dispute as the editor claims), the readers should be allowed to read upon an alternative and factually disputed assertion detailed in the website. I think, ‘Mr. Editor’ should not deprive his readers of this 'controversy' or at the very least explain why he implicitly portray (by name) Craig Mello and Andy fire as the real co-discoverers of RNAi, if the page were dedicated to a "general" discussion of RNAi. Why leave out the thousands of other equally worthy scientists who ultimately contributed to the discovery of RNAi???? Mention their name too. Let me know if you need help with names. Plus who are you...more relevantly what makes you the self-proclaimed “RNA interference historian” in any case?— Preceding unsigned comment added by Mussaali (talk • contribs) 20:32, 23 July 2006 mussa ali (UTC)
- Thanks for coming to this page to discuss these changes. Mello and Fire are the commonly recognized discoverers of RNAi and claiming otherwise based on your personal analysis or involvement with the case violates Wikipedia policies on original research and verifiability. The dispute itself seems not to be covered by any independent and reliable source outside of your website, which I'm sure you understand is not admissible as a source due to your personal involvement. Until and unless the scientific community independently recognizes other individuals as key discoverers of RNAi, the accepted attribution should remain in the article.
- Mello and Fire's labs were the two involved in the discovery, so it's not surprising that "credit" goes to them. Attribution that included every postdoc, grad student, and lab tech in either lab would be unwieldy and frankly unlikely, here or in the scientific literature. Opabinia regalis 01:14, 24 July 2006 (UTC)
Since you are the self styling RNAi historian, I think I am dying to know who you are. Are you friends of Mello and Fire? Have you done any work with RNAi before its conception. I agree with you a key recognition was published by mello and fire in 1998. And since you went to the trouble of highlighting that "historic eureka moment" any 'controvery' surrounding it is a fair game. Don't you think? Otherwise the self proclaimed historians would be having a field day and pushing a personal and biased history down everybody's throat as the true history. mussa ali
"Mello and Fire are the commonly recognized discoverers of RNAi"?? I think you should rephrase that to read as Craig and Andy are commonly extolled for having disclosed a key RNAi finding in their lab. Do you know that Craig Mello had stated publicly that he has “discarded” the original RNAi notebooks. That is a public record and verifiable. I bet in your eyes this is completely irrelevant! I will have more to say....later mussa ali— Preceding unsigned comment added by Mussaali (talk • contribs)
- I'm not an "RNAi historian", nor do I personally know Mello, Fire, or anyone else involved. I have worked a bit with RNAi in the past but don't at the moment. Also, I didn't originally add the text attributing the discovery to Mello and Fire, but since it is the accepted attribution I believe it should stay in the article. Notice that the article doesn't say anything about "eureka" moments; it only states that the two labs in collaboration were the first to notice (and publish about) dsRNA-mediated silencing in C. elegans - which is true regardless of who knew what earlier. mussa ali
- Secondly, your personal experience of what did or did not occur at the time is not an acceptable source for this article, especially if your retelling has not been published outside of your website. has there been any external, third-party coverage of this inventorship dispute? As in, a news brief in a journal about the court case(s), or something to that effect?
- (Off the subject, just FYI - you can sign your comments with four tildes (~~~~) and indent them by beginning each paragraph of your comment with a colon. Makes threaded discussions easier to read.) Opabinia regalis 06:35, 24 July 2006 (UTC)
Enough about the rnaiconception.com website issue…
Let’s look at your loose use of the word “noticed”. Since you weren’t there in either Mello’s or Fire’s lab, you do not have or ever had a first hand knowledge of who may have “noticed” it first. i.e., assuming by “noticed” you mean to infer “recognized” or “conceived.” (If you need a more direct Mello audio back up? Let me know…) mussa ali
More relevantly, (I am generously assuming that you have some RNAi background, and I encourage you to read my perspective on the competing RNAi-induction models), your use of the word “noticed” may be mechanistically flawed. Assuming Fire’s paradigm is correct, that is a dsRNA contaminant (unbeknownst to us at the time) was sufficently inducing RNAi in C. elegans, the first group to have “noticed” or observed dsRNA mediated interference in C. elegans was that of Dr. Kempheus group at Cornell in 1995. (For starters, You should hyperlink Cornell and Dr. Kempheus too). mussa ali
As such I think you should replace the word “noticed” with “reported”. mussa ali
Again you should let your readers know who you really are at least in terms of your scientific credentials and just as relevantly your affiliations(s)…..before you start deleting other people’s website with shameless pomposity. mussa ali— Preceding unsigned comment added by Mussaali (talk • contribs)
- My personal opinion is that the RNAi Wikipedia page reads more like a promotional advertisement for University of Massachusetts and the Carnegie Institution of Washington masquerading as the brief history of RNAi. You should do much better ... Mr. Historian. — Preceding unsigned comment added by 209.16.74.251 (talk • contribs) mussa ali
- FYI, and if you ever noticed, almost everything on the rnaiconception website (http://www.rnaiconception.com/inventionofrnai-2006.pdf) is supported by published documents and extensive third-party corroborations. In fact I invite or challenge you to read it thoroughly and critique it. Do not be afraid to ask me any questions you may have. Again, my gut tells me you are a spokesperson for Mello and company masquerading as an HISTORIAN. Who are you... since you are the historian, editor, site manager etc who are you???? — Preceding unsigned comment added by 70.183.113.24 (talk • contribs) mussa ali
It is time to remind the people posting here to be WP:CIVIL. Personal attacks are never appropriate.TedTalk/Contributions 00:10, 25 July 2006 (UTC)
- Well, this has exploded since I was here last. Mussa, your point about the usage of "noticed" is fair, and it's been clarified. How it can be an ad, though, is beyond me - Mello, Fire, UMass, and the Carnegie Institution collectively get one mention in one sentence at the end of a section that is primarily about plants. Opabinia regalis 00:57, 25 July 2006 (UTC)
Although I have many issues with the website, I thank you for fixing one of its major flaws. Appreciate it. — Preceding unsigned comment added by Mussaali (talk • contribs) 21:58, 24 July 2006 (UTC)
By way of closing, and assuming that the editor of this page has no ulterior motive(s) and or is not (or has ever been) C&A's friend and was not engaging in a tacit C&A promotional exercise, I want to apologize for some of the comments I have made. I think I may have gone a bit overboard. I also would like to commend the editor for the significant changes that have been made to the original version of this page, which was the genesis of my contentions.
- I can't speak for the other contributors to this article, but I don't know and have no connection with any of the people involved. I'm glad this was resolved and I'm sure you could make valuable contributions to this and related articles if you decided to stick around. Opabinia regalis 04:12, 17 August 2006 (UTC)
Mussaali I have removed the conversation on this page that had nothing to do with RNAi. If you feel the need to continue this conversation please continue on Opabinia regalis' talk page. David D. (Talk) 02:51, 30 September 2006 (UTC)
Since you won't let me respond to or edit the comments made @ http://en.wikipedia.org/wiki/User_talk:Samsara/Archive04 about me as a sign of protest I will be pasting the UMASS/sterilization audio page http://www.worcestermagazine.org/audio.pdf on any wikipedia website where UMASS or Craig Mello are mentioned.
Opabinia regalis: You have yet to reveal your identity; I am still waiting. Just your assertion alone that you are not affiliated with UMASS is not worthy of credence. For the last time, who are You? why are you afraid to tell your readers who you really are?
Question
"RNAi is distinct from other gene silencing phenomena in that silencing can spread from cell to cell and generate heritable phenotypes in first generation progeny when used in Caenorhabditis elegans." This sentence is in the introduction. There is nothing relating to this sentence in the main text so we need a section on the systemic nature of RNAi silencing. But first, what are the "other gene silencing phenomena" being referred to here? I assume X-inactivation, imprinting and heterochromatin. Or is it something else? David D. (Talk) 06:17, 26 July 2006 (UTC)
- I added a brief mention of the organisms in which RNAi is and is not systemic, but I'm not convinced the lead is really clear. I also think that, after splitting up the overview section, the new section order is a little out of whack. What do you think? Opabinia regalis 00:55, 29 July 2006 (UTC)